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Histamine synthesis by cells of the macrophage lineage in bone marrow of mice
1Nagoya University Bio-Science Center, Chikusa, Japan.
Abstract:
An injection of Escherichia coli lipopolysaccharide (LPS) increased the activity of histidine decarboxylase (HDC) in bone marrow (BM) cells of C3H/HeN mice much more than in C3H/HeJ mice, which are resistant to various effects of LPS. In WBB6/F1 (W/Wv) mice, which are genetically deficient in mast cells, HDC activity increased more than in C3H/HeN mice. Cultured BM cells of W/Wv mice spontaneously synthesized histamine in a HDC-dependent way. LPS caused a slight increase in HDC-associated histamine synthesis by these cells. Treatment of the BM cells with murine recombinant granulocyte-macrophage colony stimulating factor (mrGM-CSF) increased the histamine synthesis. In addition, treatment with mrGM-CSF made the cells respond to LPS by a dose-dependent increase in HDC activity and histamine synthesis. Most dish-adherent BM cells that had been treated with both mrGM-CSF and LPS for 48 h were stained for nonspecific esterase and not for chloroacetate esterase, and had twice as much HDC activity as the nonadherent cells had. Immunocytochemical analysis of the BM cells of W/Wv mice treated with both mrGM-CSF and LPS showed that HDC was in the cytoplasm of cells having Mac-1, a macrophage-differentiation antigen. These results suggest that cells of the macrophage lineage in the BM of mice synthesize histamine.
Insights
Bone marrow cells, particularly those of the macrophage lineage, synthesize histamine. This process is dependent on histidine decarboxylase (HDC) activity, which is influenced by lipopolysaccharide (LPS) and granulocyte-macrophage colony-stimulating factor (GM-CSF).
Area of Science:
- Immunology
- Cell Biology
- Biochemistry
Background:
- Histamine synthesis is primarily associated with mast cells and basophils.
- The role of other cell types, such as bone marrow cells, in histamine production remains less understood.
- Escherichia coli lipopolysaccharide (LPS) is known to elicit various immune responses.
Purpose of the Study:
- To investigate the role of bone marrow (BM) cells in histamine synthesis.
- To determine the involvement of histidine decarboxylase (HDC) in LPS-induced histamine production in BM cells.
- To explore the influence of murine recombinant granulocyte-macrophage colony stimulating factor (mrGM-CSF) on BM cell histamine synthesis.
Main Methods:
- Comparing HDC activity in BM cells from LPS-sensitive (C3H/HeN) and LPS-resistant (C3H/HeJ) mice.
- Assessing HDC activity and histamine synthesis in BM cells from mast cell-deficient (WBB6/F1 (W/Wv)) mice.
- Culturing BM cells with LPS and/or mrGM-CSF and measuring HDC activity and histamine synthesis.
- Characterizing BM cell populations using esterase staining and immunocytochemistry for Mac-1.
Main Results:
- LPS significantly increased HDC activity in BM cells of C3H/HeN mice compared to C3H/HeJ mice.
- BM cells from mast cell-deficient WBB6/F1 (W/Wv) mice showed higher spontaneous HDC activity and histamine synthesis.
- mrGM-CSF treatment enhanced histamine synthesis and potentiated the LPS response in BM cells.
- Adherent BM cells, identified as macrophages (Mac-1 positive), exhibited elevated HDC activity and histamine synthesis.
Conclusions:
- Bone marrow cells, specifically those of the macrophage lineage, are capable of synthesizing histamine.
- Histamine production by these cells is dependent on histidine decarboxylase (HDC) activity.
- The response of BM cells to LPS and their histamine synthesis can be modulated by mrGM-CSF, suggesting a role for macrophages in immune-related histamine release.