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Molecular species of phospholipids of interleukin-2-dependent murine cytotoxic T lymphocytes
J Nishihira1, T Ishibashi, Y Sawamura
1Department of Biochemistry, School of Medicine, Hokkaido University, Sapporo, Japan.
We reported that phospholipids of interleukin-2 (IL-2)-dependent murine cytotoxic T lymphocytes (CTL) contained high amounts of ether lipids (Biochem. Mol. Biol. Int., 33, 137-146, 1994). In this study, the molecular species of fatty acids at the sn-1 and sn-2 positions were determined. First, phosphatidylcholine mainly consisted of the 1,2-diacyl subclass (78%) in which the major molecular species was 16:0/18:1 (24%). It should be noted that arachidonic acid was abundant in both 1-O-alkyl-2-acyl subclasses in the form of 18:0/20:4 (21%) even though the content of the subclass was low (8%). Second, phosphatidylethanolamine mainly consisted of ether subclasses (93%) and contained a large amount of arachidonic acid in the forms of either 18:0/20:4 (19%) or 16:0/20:4 (10%). Third, phosphatidylinositol, a putative phospholipid involved in transmembrane signal transduction, had a significant amount of arachidonic acid at the sn-2 positions of 1,2-diacyl and 1-O-alkyl-2-acyl subclasses. Fourth, phosphatidylserine, consisting of mostly the 1-O-alkyl-2-acyl subclass (86%), contained arachidonic acid dominantly in the form of 18:0/20:4 (33%). Considering these results, it appears that CTL have the potential to mediate its signal transduction mediated by the bioactive lipids, i.e. arachidonic acid metabolites and the ether lipids.
We reported that phospholipids of interleukin-2 (IL-2)-dependent murine cytotoxic T lymphocytes (CTL) contained high amounts of ether lipids (Biochem. Mol. Biol. Int., 33, 137-146, 1994). In this study, the molecular species of fatty acids at the sn-1 and sn-2 positions were determined. First, phosphatidylcholine mainly consisted of the 1,2-diacyl subclass (78%) in which the major molecular species was 16:0/18:1 (24%). It should be noted that arachidonic acid was abundant in both 1-O-alkyl-2-acyl subclasses in the form of 18:0/20:4 (21%) even though the content of the subclass was low (8%). Second, phosphatidylethanolamine mainly consisted of ether subclasses (93%) and contained a large amount of arachidonic acid in the forms of either 18:0/20:4 (19%) or 16:0/20:4 (10%). Third, phosphatidylinositol, a putative phospholipid involved in transmembrane signal transduction, had a significant amount of arachidonic acid at the sn-2 positions of 1,2-diacyl and 1-O-alkyl-2-acyl subclasses. Fourth, phosphatidylserine, consisting of mostly the 1-O-alkyl-2-acyl subclass (86%), contained arachidonic acid dominantly in the form of 18:0/20:4 (33%). Considering these results, it appears that CTL have the potential to mediate its signal transduction mediated by the bioactive lipids, i.e. arachidonic acid metabolites and the ether lipids.