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Expression of pS2 gene in rat brain
M Hirota1, H Awatsuji, Y Sugihara
1Department of Molecular Biology, Gifu Pharmaceutical University, Japan.
Biochemistry and Molecular Biology International
|April 1, 1995
Summary
pS2 mRNA is expressed in rat brains and peripheral tissues. Its expression in the hippocampus peaks early in development, suggesting a role for pS2 protein in central nervous system development.
Area of Science:
- Neuroscience
- Molecular Biology
- Developmental Biology
Background:
- pS2 mRNA encodes a secreted protein of unknown function.
- Previous studies showed widespread pS2 mRNA expression in mice, including the brain.
Purpose of the Study:
- To investigate pS2 mRNA expression in rat brain and peripheral tissues.
- To analyze the developmental expression pattern of pS2 mRNA in the rat brain.
Main Methods:
- RT-PCR (Reverse Transcription Polymerase Chain Reaction) to detect pS2 mRNA.
- Analysis of pS2 mRNA expression in different brain regions and developmental stages in rats.
Main Results:
- pS2 mRNA was detected in the adult rat brain, primarily in the hippocampus, cerebral cortex, and cerebellum.
- Hippocampal pS2 mRNA expression peaked in 1-3 day old rats and decreased by 7 weeks.
- Midbrain pS2 mRNA levels remained low throughout the observed postnatal development.
Conclusions:
- pS2 mRNA is expressed in the developing rat central nervous system.
- The developmental expression pattern suggests pS2 protein is important for CNS development.