Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A decrease in neural sialyltransferase activity in Alzheimer's disease

T M Maguire1, K C Breen

  • 1Department of Pharmacology and Clinical Pharmacology, University of Dundee, UK.

Dementia (Basel, Switzerland)
|July 1, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Perturbations of cellular functions integral to neural tube formation by the putative teratogen sodium valproate.

Toxicology in vitro : an international journal published in association with BIBRA·2010
Same author

Glucocorticoid potentiation of lead neurotoxicity in the mouse HN9 hippocampal cell line.

Toxicology in vitro : an international journal published in association with BIBRA·2010
Same author

The amyloidogenic potential and behavioral correlates of stress.

Molecular psychiatry·2007
Same author

Ets2 transcription factor in normal and neoplastic human breast tissue.

European journal of cancer (Oxford, England : 1990)·2005
Same author

Measurement of plasminogen activator system components in plasma and tumor tissue extracts obtained from patients with breast cancer: an EORTC Receptor and Biomarker Group collaboration.

Oncology reports·2005
Same author

Overexpression of the Ets-1 transcription factor in human breast cancer.

British journal of cancer·2004
Same journal

Enhanced cytotoxic response of natural killer cells to interleukin-2 in Alzheimer's disease.

Dementia (Basel, Switzerland)·1996
Same journal

The gain of apolipoprotein E genotyping to separate patients with Alzheimer's disease from normal individuals: relevance to community studies.

Dementia (Basel, Switzerland)·1996
Same journal

Symptoms and signs in dementia: synergy and antagonism.

Dementia (Basel, Switzerland)·1996
Same journal

An algorithmic approach to the differential diagnosis of dementia.

Dementia (Basel, Switzerland)·1996
Same journal

Quantitative electroencephalography power and coherence in Alzheimer's disease and mild cognitive impairment.

Dementia (Basel, Switzerland)·1996
Same journal

Regional brain atrophy in idiopathic parkinson's disease and diffuse Lewy body disease.

Dementia (Basel, Switzerland)·1996
See all related articles

Alzheimer's disease brains show reduced sialyltransferase enzyme activity in key cortical areas. This specific biochemical change may link to neurodegeneration, excluding the hippocampus.

Area of Science:

  • Neuroscience
  • Biochemistry
  • Pathology

Background:

  • Alzheimer's disease (AD) is a progressive neurodegenerative disorder.
  • Sialyltransferases are crucial enzymes involved in glycosylation, impacting neuronal function.
  • Altered enzyme activity is implicated in AD pathogenesis.

Purpose of the Study:

  • To investigate the activity of sialyltransferase in post-mortem brain samples from Alzheimer's disease patients.
  • To compare enzyme activity in AD patients with age-matched healthy controls.
  • To determine if changes in sialyltransferase activity are specific to AD-related neurodegeneration.

Main Methods:

  • Post-mortem brain tissue samples were obtained from Alzheimer's disease patients and controls.
  • Enzyme activity assays were performed on soluble and particulate fractions of brain homogenates.

Related Experiment Videos

  • Specific brain regions (frontal cortex, temporal cortex, hippocampus) were analyzed.
  • Activity of the Golgi marker enzyme, thiamine pyrophosphatase, was measured as a control.

    • Main Results:

    • A significant decrease in both soluble and membrane-bound sialyltransferase activity was observed in the frontal and temporal cortical lobes of Alzheimer's disease brains.
    • No significant change in sialyltransferase activity was detected in the hippocampus.
    • Activity of the Golgi marker enzyme, thiamine pyrophosphatase, remained unchanged, indicating regional specificity.

    Conclusions:

    • The reduction in sialyltransferase activity in specific cortical regions is a potential biochemical hallmark of Alzheimer's disease.
    • This finding suggests a specific biochemical event linked to AD-like neurodegeneration.
    • The observed changes may contribute to the understanding of molecular mechanisms underlying Alzheimer's disease.