Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Synaptophysin immunoreactivity is stable 36 h postmortem

X Liu1, A Brun

  • 1Department of Neuropathology, Lund University, Sweden.

Dementia (Basel, Switzerland)
|July 1, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effect of dietary phosphorus and calcium depletion-repletion on pig performance and bone quality.

Animal : an international journal of animal bioscience·2026
Same author

Bones, reproductive organs and carcass characteristics of entire, early and late immunocastrated male pigs.

Animal : an international journal of animal bioscience·2025
Same author

Efficacy and safety of dupilumab in Hailey-Hailey disease: A multicentre cohort study.

Journal of the European Academy of Dermatology and Venereology : JEADV·2025
Same author

Generation and characterization of an ovine cell line derived from peripheral blood and its potential use in the study of livestock and zoonotic viral infections.

Virology journal·2025
Same author

Advances in identifying functional groups in carnosol analogues to address their efficacy in skeletal muscle function.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2025
Same author

Real-life management of psoriasis with biological agents during pregnancy.

Annales de dermatologie et de venereologie·2024
Same journal

Enhanced cytotoxic response of natural killer cells to interleukin-2 in Alzheimer's disease.

Dementia (Basel, Switzerland)·1996
Same journal

The gain of apolipoprotein E genotyping to separate patients with Alzheimer's disease from normal individuals: relevance to community studies.

Dementia (Basel, Switzerland)·1996
Same journal

Symptoms and signs in dementia: synergy and antagonism.

Dementia (Basel, Switzerland)·1996
Same journal

An algorithmic approach to the differential diagnosis of dementia.

Dementia (Basel, Switzerland)·1996
Same journal

Quantitative electroencephalography power and coherence in Alzheimer's disease and mild cognitive impairment.

Dementia (Basel, Switzerland)·1996
Same journal

Regional brain atrophy in idiopathic parkinson's disease and diffuse Lewy body disease.

Dementia (Basel, Switzerland)·1996
See all related articles

Synaptic density in human brains is reliably measured using synaptophysin immunohistochemistry within 36 hours postmortem. Synaptic density decreases with age but is unaffected by long-term tissue storage.

Area of Science:

  • Neuroscience
  • Neuropathology
  • Biomarker Research

Background:

  • Synaptic density is a crucial indicator of neuronal health and function.
  • Understanding factors affecting synaptic density measurement is vital for neuropathological studies.
  • Postmortem changes and tissue storage can potentially impact biomarker integrity.

Purpose of the Study:

  • To investigate the effects of postmortem interval, age, and tissue storage duration on synaptic density in the human prefrontal cortex.
  • To determine the reliability of synaptophysin as a marker for synaptic density under various conditions.
  • To establish optimal conditions for preserving synaptic integrity in postmortem brain tissue.

Main Methods:

  • Quantitative immunohistochemistry using a monoclonal antibody to synaptophysin.

Related Experiment Videos

  • Analysis of synaptic density in human prefrontal cortex samples from normal individuals and patients with neurological diseases.
  • Assessment of synaptophysin immunoreactivity across different postmortem intervals (1-72 h) and age ranges.

    • Main Results:

    • Synaptophysin immunoreactivity remained stable for up to 36 hours postmortem in normal cases.
    • A significant decrease in synaptophysin immunoreactivity was observed at 48 and 72 hours postmortem.
    • Synaptic density showed a significant reduction with increasing age, but was not affected by long-term storage of paraffin-embedded tissues.
    • Similar postmortem changes were noted in the disease group.

    Conclusions:

    • Synaptophysin immunohistochemistry is a valid method for measuring synaptic density in human brain tissue autopsied within 36 hours of death.
    • Long-term storage of paraffin-embedded brain tissue does not compromise synaptophysin immunoreactivity.
    • Age is a significant factor influencing synaptic density, necessitating consideration in neuropathological research.