Nutrient optimization for production of broad spectrum antibiotic by Streptomyces antibioticus SR15.4

Antibiotic production by Streptomyces antibioticus Sr15.4 was studied under various cultural conditions. During nutrient optimization it was found that the strain utilized glycerol as the best source of carbon at 1.044 molar level, and 0.020 molar arginine as the best source of nitrogen. The strain exhibited significant enhancement in antibiotic production when grown at pH 6.8.