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Quantitation of catecholamines in uninflamed human dental pulp tissues by high-performance liquid chromatography
M A Schachman1, P A Rosenberg, H A Linke
1Department of Endodontics, New York University, College of Dentistry, N.Y., USA.
Abstract:
The quantitation of catecholamines has yet to be reported in the human dental pulp. Various methods of chemical detection have shown the presence of catecholamines in pulpal tissue. Pulpal tissue was obtained from nonrestored, uninflamed teeth that needed to be extracted. The samples were collected, extracted, and analyzed by means of high-performance liquid chromatography with ultraviolet detection for levels of catecholamines (dopamine, epinephrine, and norepinephrine). As mediators of vasoconstriction, catecholamines play an important role in the control of intrapulpal pressure. This research describes a method for quantitation of catecholamines. Future studies investigating inflamed tissue can now be accomplished with the use of this analytic method. The results from this research indicate a baseline level of catecholamines in the uninflamed human dental pulp.