Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A functionally compromised intermediate in extrathymic CD8+ T cell deletion

S R Dillon1, V L MacKay, P J Fink

  • 1University of Washington, School of Medicine, Department of Immunology, Seattle 98195-7650, USA.

Immunity
|September 1, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Interleukin (IL) 31 induces in cynomolgus monkeys a rapid and intense itch response that can be inhibited by an IL-31 neutralizing antibody.

Journal of the European Academy of Dermatology and Venereology : JEADV·2016
Same author

Antigen specific suppression of T-cell responses - the veto concept.

Immunology today·2014
Same author

The transference-countertransference phenomenon: a requirement for training.

Academic psychiatry : the journal of the American Association of Directors of Psychiatric Residency Training and the Association for Academic Psychiatry·2014
Same author

The receptor tyrosine kinase MerTK regulates dendritic cell production of BAFF.

Autoimmunity·2009
Same author

TACI-Ig prevents the development of airway hyperresponsiveness in a murine model of asthma.

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology·2008
Same author

Atacicept (TACI-Ig) inhibits growth of TACI(high) primary myeloma cells in SCID-hu mice and in coculture with osteoclasts.

Leukemia·2007

Researchers identified a novel intermediate T cell population in V beta 5 TCR Tg mice undergoing peripheral deletion. These CD8lo cells are functionally compromised but not yet undergoing programmed cell death, offering insights into self-tolerance mechanisms.

Area of Science:

  • Immunology
  • T cell biology
  • Self-tolerance

Background:

  • Mature peripheral T cells must develop self-tolerance to prevent autoimmunity.
  • T cell receptor (TCR) transgenic (Tg) models are crucial for studying T cell development and tolerance induction.
  • Superantigen-induced deletion provides a model for examining T cell tolerance in vivo.

Purpose of the Study:

  • To establish a model system for analyzing self-tolerance induction in mature peripheral T cells.
  • To investigate the distinct fates of CD4+ and CD8+ T cells during superantigen-driven peripheral deletion.
  • To characterize the intermediate T cell population targeted for deletion.

Main Methods:

  • Utilized V beta 5 TCR transgenic (Tg) mice and I-E mice.
  • Administered superantigen to induce T cell deletion.

Related Experiment Videos

  • Analyzed T cell populations using flow cytometry (CD4, CD8, V beta 5 expression).
  • Assessed cell proliferation with BrdU incorporation.
  • Evaluated cell function in vitro and sensitivity to cortisone in vivo.

    • Main Results:

    • Both CD4+ and CD8+ V beta 5+ T cells underwent chronic deletion in the periphery.
    • CD4+ T cells became anergic before deletion.
    • A distinct CD8loV beta 5lo population, phenotypically similar to memory cells, emerged and incorporated BrdU rapidly.
    • Kinetics in thymectomized mice, BrdU chasing, and cortisone sensitivity indicated these CD8lo cells were targeted for deletion.
    • CD8lo cells were functionally incompetent without DNA fragmentation, indicating pre-apoptotic deletion.

    Conclusions:

    • Identified a novel intermediate T cell population (CD8lo) undergoing peripheral deletion.
    • This population is functionally compromised but has not yet initiated programmed cell death.
    • The findings provide new insights into the mechanisms of peripheral T cell tolerance and deletion pathways.