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Kinetic properties of ovine adipose tissue fructose-1,6-bisphosphatase
T C Koekemoer1, L A Stanton, W Oelofsen
1Department of Biochemistry, University of Port Elizabeth, Republic of South Africa.
Abstract:
The presence of FBPase was confirmed in both human and ovine white adipose tissue in metabolically significant amounts. The partially purified enzyme from ovine adipose tissue exhibited kinetic properties very similar to other mammalian FBPases (pH optimum of 7.5, absolute requirement for divalent metal ions and strong inhibition by both AMP and F-2,6-P2). The micromolar S0.5 value obtained suggests that the enzyme may be of physiological significance.