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HIV-plasma viral load detection by branched DNA signal amplification
R Iuliano1, G Forastieri, M Brizzi
1Department of Biomedicine, University of Pisa.
The New Microbiologica
|July 1, 1995
Abstract:
To detect HIV-1 plasma viral load in seropositive patients, we applied a new molecular quantitation technique: branched DNA signal amplification (bDNA). We performed bDNA with 99 sera from HIV-1 seropositive patients undergoing antiretroviral therapy. We compared the results obtained with p24 antigenemia and CD4+ cell count. The bDNA proved quite sensitive and available for routine use in laboratories.