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Genetic variability in different Pneumocystis isolates from man and rats
E Ortona1, P Margutti, A De Luca
1Department of Immunology, Istituto Superiore di Sanità, Roma, Italy.
The New Microbiologica
|July 1, 1995
Summary
Genetic analysis of Pneumocystis carinii reveals distinct strains in rats and humans. DNA sequencing identified mutations and deletions, supporting the existence of multiple Pneumocystis strains within host species.
Area of Science:
- Microbiology
- Genetics
- Molecular Biology
Background:
- Pneumocystis carinii is an opportunistic pathogen causing pneumonia in immunocompromised individuals.
- Understanding genetic variability is crucial for diagnosing and treating P. carinii infections.
Purpose of the Study:
- To investigate the genetic diversity among Pneumocystis carinii isolates from rat and human origins.
- To identify specific genetic markers differentiating these isolates.
Main Methods:
- Sequencing of three DNA regions: mitochondrial rRNA gene, 5S rRNA gene, and dihydrofolate reductase (DHFR) gene.
- Analysis of genetic material from P. carinii isolates obtained from immunosuppressed rats and an HIV+ patient.
Main Results:
- Point mutations and deletions were observed in the mitochondrial rRNA and 5S rRNA genes of rat isolates.
- Nucleotide sequence variations were detected in conserved genetic regions.
Conclusions:
- The observed genetic variations support the hypothesis of multiple Pneumocystis strains infecting the same host species.
- Distinct genetic profiles exist between rat and human-derived P. carinii isolates.