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Updated: Aug 7, 2026
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Discrimintion and Mapping of the Primary and Processed Transcripts in Maize Mitochondrion Using a Circular RT-PCR-based Strategy
Published on: July 29, 2019
Splicing and editing of rps10 transcripts in potato mitochondria
S Zanlungo1, V Quiñones, A Moenne
1Departamento de Biología Celular y Molecular, Facultad de Ciencias Biológicas, P. Universidad Católica de Chile, Santiago.
Current Genetics
|May 1, 1995
Summary
Potato mitochondrial ribosomal protein S10 (rps10) gene expression involves RNA editing. Editing creates functional changes and may precede splicing, impacting protein structure and gene processing.
Area of Science:
- Mitochondrial genetics
- Plant molecular biology
- RNA processing
Background:
- The potato mitochondrial rps10 gene encodes ribosomal protein S10.
- Understanding gene structure and expression is crucial for mitochondrial function.
Purpose of the Study:
- To characterize the structure and expression of the potato mitochondrial rps10 gene.
- To investigate the role and timing of RNA editing in rps10 mRNA processing.
Main Methods:
- Sequence analysis of spliced and unspliced cDNAs.
- Comparative analysis of potato and liverwort polypeptides.
Main Results:
- The rps10 gene consists of two exons and a class-II intron.
- Four C-to-U RNA editing events in the coding region alter the RPS10 polypeptide, increasing similarity to liverwort orthologs.
- One C-to-U editing event in the intron may enhance secondary structure for splicing.
- RNA editing appears to be an early step in mRNA processing, preceding splicing.
Conclusions:
- RNA editing significantly modifies the potato rps10 transcript, affecting protein sequence and potentially mRNA splicing.
- Editing is an early and essential step in the processing of mitochondrial rps10 mRNA.
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