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Formation of a mutagen, glyoxal, from DNA treated with oxygen free radicals
N Murata-Kamiya1, H Kamiya, N Iwamoto
1Department of Environmental Oncology, University of Occupational and Environmental Health, Kitakyushu, Japan.
Carcinogenesis
|September 1, 1995
Abstract:
Oxygen free radicals cause extensive chemical changes in DNA, including base and sugar modifications and strand breaks. In the present study we found that a mutagen, glyoxal, is produced by exposure of DNA to an oxygen radical forming system (5 mM FeSO4-EDTA, 37 degrees C, 60 min). It was produced with 17 times higher efficiency than 8-hydroxydeoxyguanosine. Thus it is possible that the formation of glyoxal is one of the major types of damage in DNA exposed to oxygen free radicals.