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Inhibition of degranulation of human polymorphonuclear leukocytes by complement factor D
N Balke1, U Holtkamp, W H Hörl
1Department of Biochemistry, Faculty of Chemistry, University of Bielefeld, Germany.
FEBS Letters
|September 11, 1995
Abstract:
A degranulation inhibiting protein could be isolated from human plasma ultrafiltrate by a three-step purification method including ion-exchange chromatography, gelfiltration and affinity-chromatography. The protein was identified as complement factor D by means of sequence analysis. Its degranulation inhibiting activity was determined with regard to its effect on the FNLPNTL-induced lactoferrin secretion of human polymorphonuclear leukocytes. Complement factor D caused a dose-dependent decrease of the FNLPNTL-stimulated lactoferrin degranulation down to 34% of stimulated controls.