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Mouse sperm membrane potential: changes induced by Ca2+

F Espinosa1, A Darszon

  • 1Depto. Genética y Fisiología Molecular, Universidad Nacional Autónoma de México, Morelos.

FEBS Letters
|September 18, 1995
PubMed
Summary

Mouse sperm membrane potential relies on extracellular ions like potassium, sodium, and chloride. Calcium influx triggers hyperpolarization, involving Na-K ATPase and chloride channels, potentially linking to sperm function.

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Area of Science:

  • Sperm physiology and membrane biophysics
  • Ion transport mechanisms in mammalian reproduction

Background:

  • Sperm membrane potential (Er) is crucial for sperm function and motility.
  • Extracellular ion concentrations significantly influence sperm electrophysiology.

Purpose of the Study:

  • To investigate the ionic basis of mouse sperm resting membrane potential (Er).
  • To characterize the ion transport systems involved in calcium-induced hyperpolarization (Ca-ith) in mouse sperm.
  • To explore the relationship between Ca-ith and the acrosome reaction (AR).

Main Methods:

  • Measurement of mouse sperm resting membrane potential using a potential-sensitive dye.
  • Electrophysiological studies in varying extracellular K+, Na+, Ca2+, and Cl- concentrations.
  • Pharmacological inhibition using ouabain, niflumic acid, nimodipine, trifluoperazine, and Mg2+.

Main Results:

  • Resting membrane potential (Er) depends on extracellular K+ and Na+ (in Ca2+-free media).
  • Calcium addition induces a Na+ and Cl- dependent transient hyperpolarization (Ca-ith), mimicked by Cd2+ and Mn2+.
  • Ca-ith is partially inhibited by Na-K ATPase (ouabain) and Cl- channel blockers (niflumic acid), and Ca2+ influx involves channels sensitive to nimodipine, trifluoperazine, and Mg2+.

Conclusions:

  • Mouse sperm membrane potential is regulated by extracellular ions and involves Na-K ATPase and chloride channels.
  • Calcium influx triggers a hyperpolarization dependent on Na+ and Cl-, suggesting shared transport systems with the acrosome reaction.

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