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Updated: Aug 19, 2026

Virus-induced Gene Silencing (VIGS) in Nicotiana benthamiana and Tomato
Published on: June 10, 2009
Resistance against multiple plant viruses in plants mediated by a double stranded-RNA specific ribonuclease
Y Watanabe1, T Ogawa, H Takahashi
1Department of Biosciences, School of Science and Engineering, Teikyo University, Utsunomiya, Japan.
Abstract:
Many plant viruses have single-stranded RNAs as their genomes. During the course of replication, genomic RNAs and their complementary template RNAs are likely to form double-stranded (ds) RNA at least transiently. To attack replication intermediates of many plant RNA viruses specifically, we introduced a yeast-derived ds-RNA specific RNase gene called pac 1 into plants. The transformed plants showed a decrease in lesion numbers when they were challenged with tomato mosaic virus, and a delay in the appearance of symptoms when inoculated with cucumber mosaic virus or potato virus Y.
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