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Updated: Jul 4, 2026
08:54
In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
Published on: March 29, 2019
Oligonucleotide-mediated modulation of mammalian gene expression
K J Scanlon1, Y Ohta, H Ishida
1Department of Medical Oncology, City of Hope National Medical Center, Duarte, California 91010, USA.
Summary
Oligonucleotides, including triplex DNA, antisense RNA/DNA, and ribozymes, can modulate gene expression. These molecules are increasingly explored for gene therapy and cancer treatment applications.
Area of Science:
- Molecular Genetics
- Gene Expression Regulation
Background:
- Oligonucleotides have been known to modulate gene-specific expression for over a decade.
- Advances in molecular genetics have introduced new tools like triplex DNA, antisense RNA/DNA, and ribozymes for gene manipulation.
Purpose of the Study:
- To review current knowledge on oligonucleotide-mediated gene expression modulation.
- To focus on triplex DNA, antisense oligonucleotides, and ribozymes.
- To discuss their applications in cellular signaling and potential as therapeutic agents, particularly in cancer and gene therapy.
Main Methods:
- Review of existing literature on oligonucleotide-based gene modulation.
- Focus on the biological mechanisms of triplex DNA, antisense oligonucleotides, and ribozymes.
- Analysis of their utility in research and clinical applications.
Main Results:
- Oligonucleotides are versatile tools for elucidating cellular signaling pathways.
- Antisense oligonucleotides and related technologies show promise as therapeutic agents, especially for cancer.
- Oligonucleotide-mediated gene suppression is a key strategy in emerging gene therapy approaches.
Conclusions:
- Triplex DNA, antisense oligonucleotides, and ribozymes represent significant advancements in manipulating gene expression.
- These oligonucleotides have demonstrated value in both basic research and hold substantial potential for therapeutic applications, including cancer treatment and gene therapy.
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