Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Protein kinase C involvement in apoptosis

M Lucas1, V Sánchez-Margalet

  • 1Departamento de Bioquímica Médica y Biología Molecular, Hospital Universitario Virgen Macarena, Facultad de Medicina, Sevilla, Spain.

General Pharmacology
|September 1, 1995
PubMed
Summary

Protein kinase C (PKC) involvement in apoptosis varies greatly. Its inhibition of calcium entry and interactions with sphingomyelin pathways suggest complex roles in programmed cell death and mitotic catastrophe.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Image-based Evaluation of Cutting Forces During Ultrasonic Cutting of Bone.

Experimental mechanics·2026
Same author

[Potential role of amenamevir in the management of refractory HSV-2 related acute retinal necrosis].

Journal francais d'ophtalmologie·2026
Same author

Efficacy, safety, and tolerability of arbaclofen in Autistic children and adolescents, the AIMS-2-TRIALS-CT1: a randomized, double-blind, placebo-controlled phase II trial.

EClinicalMedicine·2026
Same author

Emerging Role of MDSCS as Novel Biomarkers and Therapeutic Targets for Cancer Immunotherapy.

ImmunoTargets and therapy·2025
Same author

Characterising the SARS-CoV-2 nucleocapsid (N) protein antibody response.

The Journal of infection·2025
Same author

Quantum bath suppression in a superconducting circuit by immersion cooling.

Nature communications·2023

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • The role of Protein Kinase C (PKC) in apoptosis is complex and context-dependent, with conflicting observations across different cell types and conditions.
  • Apoptosis, or programmed cell death, can be triggered by various cellular stresses and involves intricate signaling pathways.
  • Oncogenic proteins like bcl-2 and signaling molecules such as ceramide and sphingosine are implicated in regulating cell death.

Purpose of the Study:

  • To elucidate the variable involvement of PKC in apoptosis.
  • To investigate the mechanisms by which PKC influences calcium-dependent enzymes and the sphingomyelin pathway in apoptosis.
  • To explore the role of p34cdc2 and its regulation in the context of apoptosis and mitotic catastrophe.

Main Methods:

Related Experiment Videos

  • Review and synthesis of existing literature on PKC, apoptosis, and related signaling pathways.
  • Analysis of studies investigating the effects of PKC inhibition on calcium signaling.
  • Examination of research on ceramide, sphingosine, and their impact on apoptotic pathways.
  • Investigation of the regulation of p34cdc2 by kinases and phosphatases and its relation to DNA replication.

Main Results:

  • PKC's role in apoptosis is highly variable, influenced by cell type, apoptotic stimuli, cell cycle phase, and intracellular signaling.
  • PKC inhibition of store-operated calcium entry, sensitive to bcl-2, is proposed to block calcium-dependent enzymes crucial for apoptosis.
  • The sphingomyelin pathway to apoptosis involves ceramide-activated phosphatases and PKC inhibition by sphingosine.
  • p34cdc2 is identified as a potential target protein, with its dysregulated activation relative to DNA replication leading to mitotic catastrophe, which shares features with apoptosis.

Conclusions:

  • PKC plays a multifaceted role in apoptosis, necessitating further research to understand its precise mechanisms.
  • Calcium signaling and the sphingomyelin pathway are key mediators in PKC-dependent and independent apoptotic processes.
  • The regulation of p34cdc2 is critical for preventing mitotic catastrophe and maintaining cellular integrity, highlighting its connection to apoptosis.