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Linear DNA plasmids of yeasts

H Fukuhara1

  • 1Institut Curie, Section de Biologie,Centre Universitaire Paris XI, Orsay 91405, France.

FEMS Microbiology Letters
|August 15, 1995
PubMed
Summary

Yeast linear DNA plasmids are distinct cytoplasmic genetic elements. A survey revealed their presence in 1-2% of yeast species, distinct from other organisms.

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Yeast Genetics

Background:

  • Linear DNA plasmids are found in fungi and plants, typically mitochondrial.
  • Yeast linear DNA plasmids exhibit unique cytoplasmic localization and genetic organization, differentiating them from other plasmid types.

Purpose of the Study:

  • To survey the prevalence and characteristics of plasmids across a broad range of yeast species.
  • To investigate the nature and frequency of linear DNA plasmids in yeast.

Main Methods:

  • Conducted a comprehensive survey of approximately 1800 yeast strains, representing about 600 species.
  • Analyzed plasmid DNA, focusing on the identification and characterization of linear DNA plasmids.

Main Results:

  • Linear DNA plasmids were identified in 1-2% of surveyed yeast strains, with over 20 new instances discovered across diverse species.
  • Circular DNA plasmids were found to be restricted to specific yeast genera and species.
  • The identified linear DNAs share common features with linear killer DNAs from Kluyveromyces lactis, suggesting a related family of plasmids.

Conclusions:

  • Yeast linear DNA plasmids represent a distinct group with unique cytoplasmic properties and genetic organization.
  • These plasmids are widespread among yeast species, forming a large family of small, multicopy DNA elements.
  • The findings contribute to understanding the diversity and evolution of plasmids in eukaryotic microorganisms.

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