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Strain typing of Lentinula edodes by random amplified polymorphic DNA assay
1Mycology and Botany Department, American Type Culture Collection, Rockville, MD 20852-1776, USA.
FEMS Microbiology Letters
|August 15, 1995
Summary
Randomly amplified polymorphic DNA (RAPD) markers effectively distinguished 13 of 15 shiitake mushroom strains. This DNA fingerprinting technique shows promise for shiitake breeding and strain improvement programs.
Area of Science:
- Mycology
- Molecular Biology
- Genetics
Background:
- Lentinula edodes, commonly known as shiitake mushroom, is an economically important edible fungus.
- Efficient methods for strain identification and characterization are crucial for its cultivation and breeding.
Purpose of the Study:
- To develop and evaluate randomly amplified polymorphic DNA (RAPD) markers for strain differentiation in Lentinula edodes.
- To assess the potential of RAPD markers in shiitake mushroom breeding and strain improvement.
Main Methods:
- DNA was extracted from 15 different strains of Lentinula edodes.
- Randomly amplified polymorphic DNA (RAPD) analysis was performed using single 10-base primers.
- Amplification products were separated by gel electrophoresis to visualize DNA polymorphisms.
Main Results:
- Seven out of the tested primers successfully generated polymorphic DNA markers.
- Each primer produced 12-19 distinct DNA bands ranging from 0.34 to 2.52 kb.
- Unique DNA fingerprints were observed in 13 of the 15 strains, while two strains (ATCC 28759 and ATCC 28760) showed identical RAPD profiles.
Conclusions:
- RAPD markers are effective in distinguishing between different strains of Lentinula edodes.
- The developed molecular-genetic markers have significant potential for application in shiitake mushroom breeding and strain improvement programs.
