Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A Cryptosporidium parvum genomic region encoding hemolytic activity

M I Steele1, T L Kuhls, K Nida

  • 1Department of Pediatrics, University of Oklahoma Health Sciences Center, Oklahoma City 73126, USA.

Infection and Immunity
|October 1, 1995
PubMed
Summary

Researchers identified a Cryptosporidium parvum protein, hemA, essential for host cell invasion. This protein

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genetic homogeneity of the invasive lionfish across the Northwestern Atlantic and the Gulf of Mexico based on Single Nucleotide Polymorphisms.

Scientific reports·2018
Same author

Fine-scale genetic structure due to adaptive divergence among microhabitats.

Heredity·2017
Same author

Genomic variation among populations of threatened coral: Acropora cervicornis.

BMC genomics·2016
Same author

Population Genomics of the Euryhaline Teleost Poecilia latipinna.

PloS one·2015
Same author

Pulmonary lesions and clinical disease response to Mannheimia haemolytica challenge 10 days following administration of tildipirosin or tulathromycin.

Journal of animal science·2013
Same author

Cellulose and lignin degradation in forest soils: Response to moisture, temperature, and acidity.

Microbial ecology·2013

Area of Science:

  • Parasitology
  • Molecular Biology
  • Cell Biology

Background:

  • Cryptosporidium parvum infection requires host and parasite cell membrane disruption for invasion and development.
  • Identifying parasite proteins involved in membrane alteration is crucial for understanding pathogenesis.

Purpose of the Study:

  • To screen for Cryptosporidium parvum proteins involved in host cell membrane disruption.
  • To identify and characterize a specific protein responsible for hemolytic activity.

Main Methods:

  • Screening of a C. parvum genomic expression library using hemolytic activity as a marker.
  • Mapping of hemolytic activity to a specific gene (hemA) and characterization of its encoded protein.
  • Analysis of hemA transcription in different life cycle stages and infected hosts.

Related Experiment Videos

Main Results:

  • A hemolytic clone (H4) containing a 5.5-kb genomic fragment was identified.
  • Hemolytic activity was mapped to a 690-bp open reading frame, hemA, encoding a 21-kDa protein.
  • hemA was found to be actively transcribed in excysted sporozoites and in infected mouse intestines.

Conclusions:

  • The hemA gene encodes a protein crucial for Cryptosporidium parvum's hemolytic activity.
  • This protein likely plays a significant role in the membrane disruptions necessary for parasite invasion and development.
  • hemA represents a potential target for therapeutic interventions against cryptosporidiosis.