Related Experiment Videos
Primary structure of a cytotoxin-like basic protein from Naja naja naja (Indian cobra) venom
A S Babu1, K D Puri, T V Gowda
1Department of Studies in Biochemistry, University of Mysore, Manasagangotri, India.
International Journal of Peptide and Protein Research
|July 1, 1995
Abstract:
The complete amino acid sequence of a cytotoxin-like basic protein (CLBP) from the venom of Naja naja naja (Indian Cobra) was determined by manual degradation using a 4-dimethylaminoazobenzene-4'-isothiocyanate double-coupling method. Peptide fragments obtained by chemical cleavage with cyanogen bromide and enzymic cleavages with trypsin and Staphylococcus aureus proteases for sequence analysis were purified by reversed-phase chromatography. The total number of amino acid residues was 61, with leucine as the C-terminal residue.