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Cloning and in vivo expression of the pig MyoD gene
K C Chang1, K Fernandes, P D Chantler
1Department of Veterinary Basic Sciences, Royal Veterinary College, University of London, UK.
Journal of Muscle Research and Cell Motility
|June 1, 1995
Abstract:
The complete sequence of the pig MyoD gene has been determined through the isolation and characterization of a 17 kb genomic clone. The deduced amino acid sequence shows relative conservation of about 90% with the MyoD sequences determined in other mammalian species, though there are several non-conservative changes scattered throughout the C-terminus. In situ hybridization on pig embryos (20-30 days post coitum) showed that MyoD expression was confined to myotomes and skeletal muscle masses.