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Tissue analysis of plasticizer in dogs
Proceedings of the Clinical Dialysis and Transplant Forum
|November 18, 1978
Summary
This study shows that the plasticizer DEHP is excreted by the kidneys and can accumulate in tissues like the lungs. Further research is underway to identify its metabolites.
Area of Science:
- Toxicology
- Environmental Health
- Analytical Chemistry
Background:
- Di(2-ethylhexyl) phthalate (DEHP) is a widely used plasticizer with potential health implications.
- Understanding DEHP's metabolic fate and excretion pathways is crucial for risk assessment.
- Kidney function plays a significant role in the elimination of xenobiotics.
Purpose of the Study:
- To develop analytical methods for quantifying DEHP in canine urine and tissues.
- To investigate the excretion and tissue distribution of DEHP following acute renal failure.
- To explore potential DEHP metabolism and tissue uptake mechanisms.
Main Methods:
- Induction of acute renal failure in a canine model.
- Development and application of gas chromatography for DEHP analysis in biological samples.
- Comparative analysis of DEHP levels in serum and various tissues (kidney, liver, brain, heart, lung) across different experimental groups.
Main Results:
- DEHP was detected in the urine of control and sham-operated dogs, confirming renal excretion.
- Nephrectomized dogs showed a delayed increase in serum DEHP levels.
- Lung tissue exhibited the highest DEHP concentration across all groups, with elevated levels in nephrectomized dogs compared to controls.
Conclusions:
- The kidneys play a significant role in the excretion of DEHP.
- DEHP can be metabolized or taken up by tissues, as suggested by serum level dynamics and tissue distribution.
- Lung tissue is a primary site for DEHP accumulation.