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Cytotoxic interactive effects of dentin bonding components on mouse fibroblasts
S Ratanasathien1, J C Wataha, C T Hanks
1Oral Health Sciences Program, University of Michigan, School of Dentistry, Ann Arbor 48109-1078, USA.
Journal of Dental Research
|September 1, 1995
Summary
Dentin bonding agents can be toxic due to component interactions. Longer exposure times and combined effects of HEMA, Bis-GMA, TEGDMA, and UDMA significantly increase cytotoxicity, impacting in vivo safety.
Area of Science:
- Dental Materials Science
- Biomaterials
- Toxicology
Background:
- Dentin bonding systems (DBS) exhibit variable pulpal responses.
- In vitro and in vivo toxicity of DBS show poor correlation.
- DBS components may diffuse through dentin, causing cytotoxicity via interactive effects.
Purpose of the Study:
- To investigate the individual cytotoxicities of four DBS components: HEMA, Bis-GMA, TEGDMA, and UDMA.
- To evaluate the interactive cytotoxic effects of three binary combinations of these components.
- To determine the influence of exposure time on DBS component cytotoxicity.
Main Methods:
- Cytotoxicity assessed using the MTT assay on Balb/c 3T3 mouse fibroblasts.
- Determined concentrations causing 50% toxicity (TC50 values) for individual components and combinations.
- Analyzed interactive effects (synergism, additivism, antagonism) based on observed vs. expected MTT activities.
Main Results:
- Toxicity ranking: Bis-GMA > UDMA > TEGDMA >>> HEMA.
- Binary combinations exhibited synergism, additivism, and antagonism, influenced by component concentrations.
- Increased exposure time (24 vs. 72 hours) significantly enhanced cytotoxicity for components and combinations.
Conclusions:
- Exposure time and component interactions are critical factors in DBS cytotoxicity.
- Findings suggest complex interactions influence the in vivo safety of dentin bonding agents.
- Further research needed to understand synergistic and antagonistic effects for improved DBS formulation.