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Isolation and preliminary characterization of cDNA encoding American cockroach allergens
1Department of Medical Research, Taichung Veterans General Hospital, Taiwan, Republic of China.
The Journal of Allergy and Clinical Immunology
|September 1, 1995
Summary
Researchers identified key American cockroach allergens using cDNA cloning. These recombinant allergens show promise for diagnosing and treating cockroach allergy.
Area of Science:
- Molecular Biology
- Immunology
- Entomology
Background:
- American cockroach (Periplaneta americana) allergens are crucial for understanding allergic reactions.
- Two major allergens, 78 and 72 kDa proteins in Cr-PI, have been identified.
Purpose of the Study:
- To isolate and characterize cDNA clones encoding major American cockroach allergens.
- To evaluate the potential of recombinant allergens for diagnostic and therapeutic applications.
Main Methods:
- A lambda gt22A cDNA library from American cockroach mRNA was screened using antisera.
- Positive clones were further analyzed using monoclonal antibodies and IgE from atopic sera.
- Selected clones were expressed in E. coli, and fusion proteins were analyzed via immunoblotting.
Main Results:
- Eleven cDNA clones reactive with IgE antibodies were isolated.
- Fusion proteins of 78, 72, 54, and 46 kDa were identified from selected clones.
- These fusion proteins demonstrated binding to human IgE antibodies, confirming their allergenic potential.
Conclusions:
- The identified cDNA clones likely code for major American cockroach allergens.
- Recombinant allergens derived from these clones hold significant potential for allergy diagnostics and therapeutics.
