Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Thyrotropin binding specificity for the thyrotropin receptor

Y Nagayama1, H Yamasaki, A Takeshita

  • 1First Department of Internal Medicine, Atomic Disease Institute, Nagasaki University School of Medicine, Japan.

Journal of Endocrinological Investigation
|April 1, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Molecular mechanism involved in arteriosclerosis--the roles of nitric oxide and Rho/Rho-kinase].

Nihon Naika Gakkai zasshi. The Journal of the Japanese Society of Internal Medicine·2001
Same author

Adenovirus-mediated transfer of dominant-negative rho-kinase induces a regression of coronary arteriosclerosis in pigs in vivo.

Arteriosclerosis, thrombosis, and vascular biology·2001
Same author

Regression by ACE inhibition of arteriosclerotic changes induced by chronic blockade of NO synthesis in rats.

American journal of physiology. Heart and circulatory physiology·2001
Same author

Glutamate release via NO production evoked by NMDA in the NTS enhances hypotension and bradycardia in vivo.

American journal of physiology. Regulatory, integrative and comparative physiology·2001
Same author

Involvement of Rho-kinase in hypertensive vascular disease: a novel therapeutic target in hypertension.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2001
Same author

Activating mutation of D835 within the activation loop of FLT3 in human hematologic malignancies.

Blood·2001

Thyroid-stimulating hormone (TSH) specificity was re-evaluated. Highly purified bovine TSH (bTSH) activated the LH/choriogonadotropin receptor (LH/CGR) due to luteinizing hormone (LH) contamination, not TSH itself.

Area of Science:

  • Endocrinology
  • Molecular Biology
  • Receptor Pharmacology

Background:

  • Recent studies suggested bovine TSH (bTSH) and recombinant human TSH (hTSH) activate the LH/choriogonadotropin receptor (LH/CGR).
  • These findings challenged the established concept of TSH receptor specificity.
  • This necessitated a re-evaluation of TSH and LH/CGR interactions.

Purpose of the Study:

  • To re-evaluate the interaction between TSH and the LH/CGR.
  • To determine if TSH or contaminants activate the LH/CGR.
  • To assess TSH receptor (TSHR) specificity.

Main Methods:

  • Utilized Chinese hamster ovary (CHO) cells stably expressing recombinant human TSHR and rat LH/CGR.
  • Assessed intracellular cAMP levels in response to partially and highly purified bTSH, and recombinant hTSH.

Related Experiment Videos

  • Quantified TSH binding affinity (Kd) to CHO-LH/CGR and CHO-TSHR cells.

    • Main Results:

    • Partially and highly purified bTSH increased cAMP in CHO-LH/CGR cells, indicating LH contamination.
    • Recombinant hTSH did not activate CHO-LH/CGR cells.
    • TSH preparations showed specific binding to CHO-TSHR cells but only non-specific binding to CHO-LH/CGR cells.

    Conclusions:

    • Highly purified bTSH contains significant LH contamination, explaining its reported LH/CGR activation.
    • TSH itself does not appear to activate the LH/CGR.
    • These findings reaffirm the specificity of TSH for its own receptor.