Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Changes in integrin expression during chondrogenesis in vitro: an immunomorphological study

M Shakibaei1, B Zimmermann, H J Merker

  • 1Institute of Anatomy, Free University of Berlin, Germany.

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
|October 1, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Assessment of degenerative states of articular cartilage via sparse laser-based mid-infrared attenuated total reflectance spectroscopy.

Osteoarthritis and cartilage open·2026
Same author

Ongoing multiparameter unrest at the Montagne Pelée volcano on Martinique from 2019 to 2024.

Scientific reports·2025
Same author

Contrasting risk patterns from human hunters and a large carnivore influence the habitat selection of shared prey.

Oecologia·2025
Same author

Laser-irradiating infrared attenuated total reflection spectroscopy of articular cartilage: Potential and challenges for diagnosing osteoarthritis.

Osteoarthritis and cartilage open·2024
Same author

Infrared spectroscopy is suitable for objective assessment of articular cartilage health.

Osteoarthritis and cartilage open·2022
Same author

Tularemia: A rare cause of pediatric lymph nodes adenitis.

Archives de pediatrie : organe officiel de la Societe francaise de pediatrie·2021

Integrins, such as alpha 1- and alpha 3-integrins, change during cartilage development. This study shows integrin expression correlates with collagen type during chondrogenesis and dedifferentiation.

Area of Science:

  • Cell Biology
  • Developmental Biology
  • Biochemistry

Background:

  • Integrins are crucial cell surface receptors mediating cell-cell and cell-matrix interactions.
  • Specific integrin alpha and beta chains bind to distinct ligands, influencing cellular behavior.
  • Chondrogenesis involves mesenchymal stem cell differentiation into chondrocytes, producing cartilage-specific extracellular matrix.

Purpose of the Study:

  • To investigate the dynamic expression patterns of alpha 1- and alpha 3-integrins during chondrogenesis in vitro.
  • To correlate integrin expression with the synthesis of collagen Types I and II in developing cartilage.
  • To understand the role of integrins in the differentiation and dedifferentiation processes of chondrocytes.

Main Methods:

  • Organ culture of mouse embryonic mesenchymal cells.

Related Experiment Videos

  • Immunofluorescence and immunoelectron microscopy techniques.
  • Analysis of integrin and collagen distribution at various culture time points.

    • Main Results:

    • Alpha 1-integrin was detected early in culture, while alpha 3-integrin appeared with cartilage matrix formation.
    • Differential expression of alpha 1 and alpha 3 integrins was observed in cartilage nodules, perichondrium, and surrounding mesenchyme.
    • A shift in integrin expression from alpha 1 to alpha 3 accompanied the switch from collagen Type I to Type II synthesis during chondrogenesis.

    Conclusions:

    • Integrin expression dynamically changes during chondrogenesis, correlating with collagen type synthesis.
    • Alpha 1-integrin is associated with collagen Type I and potentially involved in dedifferentiation.
    • Alpha 3-integrin expression is linked to collagen Type II and cartilage formation, suggesting a strict receptor-matrix correlation.