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IgG subtype is correlated with efficiency of passive protection and effector function of anti-herpes simplex virus
S T Ishizaka1, P Piacente, J Silva
1Department of Viral Vaccine Research and Development, Lederle-Praxis Biologicals, Pearl River, New York 10965, USA.
Abstract:
IgG subclasses differ in their effector functions in a variety of in vitro assays. To assess the effect of antibody subclass differences on in vivo protective efficacy against herpes simplex virus (HSV), a series of subclass switch mutants was made from an anti-HSV glycoprotein D monoclonal antibody. Purified antibody was examined for the ability to protect against HSV-2 challenge in mice. IgG2a was found to be more effective than IgG1. This correlated both with activity in antibody-dependent cell-mediated cytotoxicity and with efficiency of complement-mediated neutralization. These data suggest that optimization of passive immunization against HSV requires consideration of antibody subclass.