Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Neutrophil functional responses depend on immune complex valency

G R Strohmeier1, B A Brunkhorst, K F Seetoo

  • 1Department of Biochemistry, Boston University School of Medicine, Massachusetts 02118, USA.

Journal of Leukocyte Biology
|October 1, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evidence of Electron Heating by Alpha Particles in JET Deuterium-Tritium Plasmas.

Physical review letters·2023
Same author

Scaling Up Latent Tuberculosis Infection Testing and Treatment for Non-US Born Patients in a Federally Qualified Community Health Center.

Journal of immigrant and minority health·2023
Same author

Decreasing time spent in airborne infection isolation during TB testing.

The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease·2021
Same author

JET diagnostic enhancements testing and commissioning in preparation for DT scientific campaigns.

The Review of scientific instruments·2018
Same author

Ion temperature and toroidal rotation in JET's low torque plasmas.

The Review of scientific instruments·2016
Same author

MHD marking using the MSE polarimeter optics in ILW JET plasmas.

The Review of scientific instruments·2016
Same journal

Correction to: IL-27 signaling negatively regulates FcɛRI-mediated mast cell activation and allergic response.

Journal of leukocyte biology·2026
Same journal

Autofluorescence enables detection and quantification of EETosis in human leukocyte cultures.

Journal of leukocyte biology·2026
Same journal

Cold Atmospheric Plasma as an Immunomodulator: Suppression of T-cell Hyperactivation and Graft-versus-Host Disease via Redox Regulation.

Journal of leukocyte biology·2026
Same journal

Distinct lymphocyte immune signatures to nivolumab and recombinant IL-7 ex vivo in patients with sepsis.

Journal of leukocyte biology·2026
Same journal

Eosinophil Essentials: Identification and Assessment of Activity.

Journal of leukocyte biology·2026
Same journal

Immune cell landscape reveals 5 immune-related subtypes and molecular characteristics with prognostic and therapeutic implications in pan-cancer.

Journal of leukocyte biology·2026
See all related articles

Fc gamma receptor (Fc gamma R) cross-linking on neutrophils is crucial for triggering effector functions like oxidative burst. While signaling responses occur with Fc gamma R occupancy, full activation requires cross-linking.

Area of Science:

  • Immunology
  • Cellular Biology
  • Neutrophil Function

Background:

  • Neutrophil activation is critical for immune responses.
  • Fc gamma receptors (Fc gamma R) mediate neutrophil stimulation through immune complex (IC) binding.
  • The role of Fc gamma R cross-linking valency in neutrophil activation requires further elucidation.

Purpose of the Study:

  • To investigate the distinct roles of low valency immune complexes (LICs) and high valency immune complexes (HICs) in neutrophil activation.
  • To determine the necessity of Fc gamma R cross-linking for specific neutrophil effector functions.
  • To correlate Fc gamma R occupancy with signaling events and effector functions.

Main Methods:

  • Utilized multiparameter flow cytometry to simultaneously measure Ca2+ flux, oxidative burst, and membrane potential in neutrophils.

Related Experiment Videos

  • Compared neutrophil responses to LICs, HICs, and in situ cross-linked LICs (L/Ab).
  • Assessed azurophilic degranulation via elastase release.

    • Main Results:

    • Fc gamma R cross-linking, induced by HICs or L/Ab, was essential for initiating the oxidative burst and membrane potential changes.
    • LICs alone induced minimal intracellular pH changes and did not trigger oxidative burst or membrane potential changes without cross-linking.
    • Azurophilic degranulation was observed with HIC stimulation but not with L/Ab-induced cross-linking, indicating differential requirements for Fc gamma R cross-linking.
    • Ca2+ responses were dependent on dose for LICs but independent for HICs, with L/Ab eliciting similar Ca2+ responses to HICs.

    Conclusions:

    • Neutrophil effector functions like oxidative burst and azurophilic degranulation necessitate Fc gamma R cross-linking.
    • Signaling events, including Ca2+ flux, intracellular pH, and membrane potential changes, can be initiated by Fc gamma R occupancy alone, but are more robust and rapid with cross-linking.
    • The valency of immune complexes significantly influences the downstream signaling pathways and effector functions of neutrophils.