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Immunoenzymatic methods applied to the search for bioactive taxoids from Taxus baccata
Y Guo1, R Vanhaelen-Fastré, B Diallo
1Laboratory of Pharmacognosy and Bromatology, Université Libre de Bruxelles, Belgium.
Journal of Natural Products
|July 1, 1995
Abstract:
Polyclonal antibodies raised against 2'-succinyltaxol-bovine serum albumin (BSA) conjugate were used for the immunodetection of bioactive taxoids in chromatographic fractions of the stem bark extract of Taxus baccata. In addition to taxol, cephalomannine, and baccatin III, two taxoids were isolated and their structures were elucidated as 4 alpha,7 beta-diacetoxy-2 alpha,9 alpha-dibenzoxy-5 beta,20-epoxy-10 beta, 13 alpha, 15-trihydroxy-11(15-->1)-abeo-tax-11-ene[5] and taxol C [6] using spectroscopic methods.