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Expression of CD60 on multiple cell lineages in inflammatory synovitis
1Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, USA.
Laboratory Investigation; a Journal of Technical Methods and Pathology
|September 1, 1995
Summary
CD60, a T cell marker, is broadly expressed on synovial tissue cells, not just lymphocytes. This suggests CD60 may play a role in activating various cell types within the synovium.
Area of Science:
- Immunology
- Cell Biology
- Rheumatology
Background:
- CD60 is a T cell marker found on most T lymphocytes in synovial fluid and tissue.
- T cell activation can be triggered via CD60.
- CD60 is also present on neuroectodermally derived cells in the thymus and skin.
Purpose of the Study:
- To investigate the expression and distribution of CD60 in synovial tissue.
- To determine if CD60 is expressed by non-lymphoid cells in the synovium.
- To explore the potential functional role of CD60 in synovial tissue.
Main Methods:
- Immunohistologic analysis of formalin-fixed synovial and thymus tissue samples.
- Flow cytometry analysis of nonlymphoid cell lines from similar tissues.
Main Results:
- CD60 is readily identified in formalin-fixed, paraffin-embedded tissues.
- CD60 is expressed by synovial lining cells, vascular endothelium, and dendritic cells, in addition to T cells.
- CD60 expression in synovium is similar in rheumatoid arthritis and other inflammatory arthritis, and strongly expressed by giant cells in pigmented villonodular synovitis.
Conclusions:
- CD60 is a broadly distributed antigen in synovial tissue with a potential functional role in activating diverse cell populations.
- CD60 may serve as a marker for novel cell subsets in the synovial compartment, potentially of neuroectodermal origin.