[Suckling mouse brain rabies vaccine supplemented with an adjuvant. Its use in cattle]

This paper describes two experiments carried out in cattle immunized with suckling mouse brain rabies vaccine (SMB) 14, supplemented with 2% Al (OH)3 15 or Freund's modified incomplete adjuvant 2, 26. When determining the vaccinal dose, it was observed that the immune response was independent from the doses used (Table 1), all animals survived the challenge 30 days after vaccination, as compared to a mortality rate of 80% in the controls. To determine the duration of immunity, an amount of 5 ml was chosen as vacinal dose. Two years after immunization, both vaccines protected 96% of the cattle against a viral challenge that killed 63% of the non-vaccinated controls (Table 2). Statistically significant differences were observed between the antibody levels elicited by both vaccines. Antibody levels observed with the oil supplemented vaccine were higher than those produced by the A1 (OH)3 vaccine.