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Updated: Aug 23, 2026

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Purification of Native Complexes for Structural Study Using a Tandem Affinity Tag Method
Published on: July 27, 2016
Neurospora crassa glutamine synthetase. Purification by affinity chromatography and characterization of subunit
The Journal of Biological Chemistry
|August 10, 1976
Abstract:
Neurospora crassa glutamine synthetase was purified to homogeneity by a procedure based on affinity chromatography. The enzyme is adsorbed to a matrix of anthranilic acid bound to Sepharose and eluted with AMP. Different experimental approaches indicate that the enzyme has an octameric structure formed by subunits of identical molecular weight.

