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Novel insertion sequence-like element IS982 in lactococci

W Yu1, I Mierau, A Mars

  • 1Department of Food Science and Nutrition, University of Minnesota, St. Paul 55108, USA.

Plasmid
|May 1, 1995
PubMed
Summary
This summary is machine-generated.

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A novel insertion sequence-like element, IS982, was identified in Lactococcus lactis. This element, found on a lactose plasmid and chromosome, may represent a new family of mobile genetic elements.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Lactococcus lactis is a key bacterium in dairy fermentation.
  • Mobile genetic elements, such as insertion sequences (IS), play crucial roles in bacterial genome evolution.
  • Understanding IS elements is vital for comprehending genetic diversity and adaptation in bacteria.

Purpose of the Study:

  • To characterize a novel insertion sequence-like element, IS982, identified in Lactococcus lactis.
  • To investigate the distribution and potential evolutionary relationships of IS982 within lactococcal strains.

Main Methods:

  • DNA sequencing to determine the full length and open reading frame of IS982.
  • Comparative sequence analysis to identify homologous elements.
  • Southern blot analysis to assess the copy number and distribution of IS982 in different Lactococcus lactis strains.

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Main Results:

  • A novel 1003-bp insertion sequence-like element, IS982, was identified on the lactose plasmid pSK11L of Lactococcus lactis subsp. cremoris SK11.
  • IS982 possesses perfect inverted repeats and encodes a putative transposase.
  • A highly similar element was found on the chromosome of Lactococcus lactis subsp. cremoris Wg2.
  • IS982 was detected in multiple lactococcal strains with varying copy numbers.
  • IS982 shows sequence homology to RSBst-alpha from Bacillus stearothermophilus, suggesting a potential new family of IS elements.

Conclusions:

  • IS982 is a novel insertion sequence-like element found in Lactococcus lactis.
  • The presence of IS982 on both plasmids and chromosomes suggests its mobility within the bacterial genome.
  • IS982 and RSBst-alpha may constitute a distinct family of insertion sequence elements, expanding our knowledge of bacterial mobile genetic elements.