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Related Experiment Videos

Progress and emerging problems in livestock transgenesis: a summary perspective

R F Seamark1

  • 1Department of Obstetrics and Gynaecology, University of Adelaide, Australia.

Reproduction, Fertility, and Development
|January 1, 1994
PubMed
Summary

Generating transgenic livestock is advancing with embryonic stem (ES) cell technology and nucleus transfer. These methods promise more precise gene transfer for livestock breeding, overcoming current limitations.

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Area of Science:

  • Animal Biotechnology
  • Genetics
  • Reproductive Biology

Background:

  • Transgenic livestock creation is complex, with microinjection to zygotes showing limited efficiency and precision for breeding.
  • Embryonic stem (ES) cell lines offer a promising, adaptable approach for precise gene manipulation in livestock genomes.
  • Current ES cell lines for livestock lack demonstrated totipotency, hindering widespread application.

Purpose of the Study:

  • To review advancements in transgenic livestock technology.
  • To highlight the potential of ES cell-based and nucleus transfer methods.
  • To assess the future applicability of transgenesis in livestock breeding.

Main Methods:

  • Review of current gene transfer techniques, including microinjection.

Related Experiment Videos

  • Exploration of embryonic stem (ES) cell-based strategies for livestock.
  • Evaluation of nucleus transfer (cloning) technologies for livestock transgenesis.

    • Main Results:

    • The chimaeric route for ES cell genome reinstatement into the pig germ line is a significant development.
    • Nucleus transfer (cloning) shows promise for livestock with long generation times and single offspring.
    • ES cell technology is progressing but totipotency in livestock remains unproven.

    Conclusions:

    • Embryonic stem (ES) cell technology and nucleus transfer offer optimistic future solutions for livestock transgenesis.
    • Precise gene transfer in livestock breeding is becoming more feasible with new technologies.
    • Potential challenges, such as tissue culture effects on imprinting, require further investigation for predictable outcomes.