Related Experiment Videos
FFA-1, a protein that promotes the formation of replication centers within nuclei
1University of California, San Diego, Department of Biology, La Jolla, CA 92093, USA.
Science (New York, N.Y.)
|September 29, 1995
Abstract:
In the nuclei of eukaryotic cells, initiation of DNA replication occurs at a discrete number of foci. One component of these foci is the DNA replication factor RP-A. Here, the process leading to the association of RP-A with foci was reconstituted with cytosolic fractions derived from Xenopus eggs. With the use of this fractionated system, a 170-kilodalton protein required for the assembly of RP-A into foci was identified and purified. The protein appears to be an integral component of the foci at which replication of DNA is initiated in eukaryotic nuclei.