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Related Experiment Videos

Amino acid uptake in ischemically compromised brain tissue

A Jacobs1

  • 1Max-Planck-Institut für neurologische Forschung and Neurologische Universitätsklinik Köln, Germany.

Stroke
|October 1, 1995
PubMed
Summary

Multitracer positron emission tomography (PET) revealed mild increases in L-[11C]methylmethionine (11C-MET) uptake around brain infarcts. This amino acid accumulation may indicate blood-brain barrier breakdown or adaptive changes in surviving tissue.

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Area of Science:

  • Neuroimaging
  • Neurology
  • Radiochemistry

Background:

  • Focal ischemia, or stroke, disrupts normal brain function.
  • Understanding tissue changes around the infarct is crucial for prognosis.

Purpose of the Study:

  • To investigate local amino acid accumulation in brain tissue surrounding focal ischemia using multitracer positron emission tomography (PET).

Main Methods:

  • Employed PET with oxygen-15 labeled water/oxygen for measuring cerebral metabolic rate of oxygen (CMRO2) and cerebral blood flow (CBF).
  • Utilized carbon-15 labeled carbon monoxide for blood volume and oxygen extraction fraction calculations.
  • Assessed amino acid accumulation using L-[11C]methylmethionine (11C-MET) in 14 acute ischemic stroke patients across two PET studies.

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Main Results:

  • Initially increased 11C-MET uptake was observed in peri-infarct areas, correlating positively with improved CMRO2 over time.
  • Peri-infarct 11C-MET uptake negatively correlated with oxygen extraction fraction and positively with CBF.
  • Initial 11C-MET uptake correlated with follow-up CMRO2 in both infarcted and peri-infarct regions.

Conclusions:

  • Mild focal increases in 11C-MET uptake were observed in peri-infarct brain tissue.
  • These increases may signify blood-brain barrier breakdown with poor prognosis or adaptive changes in surviving tissue with a favorable prognosis.