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Rhinosporodiosis. Diagnosis by scrape cytology
M M Kamal1, A S Luley, S G Mundhada
1Department of Pathology, Government Medical College, Nagpur, Maharashtra, India.
Objective:
To determine the utility of the simple technique of scrape cytology for obtaining a morphologic diagnosis of Rhinosporodium seeberi.
Study Design:
The study included nine patients presenting with a nasal polyp alone and one patient presenting with a skin ulcer with a recurrent nasal polyp. Scrape smears from all 10 nasal polyps and skin ulcers were collected. Periodic acid-Schiff (PAS) stain was used on smears from three cases. Histopathology of all the nasal polyps and the excised skin ulcer was done.
Results:
The scrape smears showed characteristic spores of R seeberi mixed with an acute and chronic inflammatory exudate and metaplastic cells. PAS stain showed positivity for the capsule. Histopathology confirmed the cytologic diagnosis.
Conclusion:
Since mature sporangia rupture either on the surface or within the mucosa of polyps, scrape smears reliably and safely retrieve abundant spores. Therefore, this technique, offers immense potential for a rapid and correct preoperative diagnosis of rhinosporodiosis. It thereby facilitates the planning of surgical interventions to prevent recurrences.