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Related Experiment Videos

Ovarian levels of epidermal growth factor receptor mRNA in the rat--a postovulatory decrease

B Assarsson1, I Nilsson, G Selstam

  • 1Department of Physiology, University of Umeå, Sweden.

Acta Physiologica Scandinavica
|June 1, 1995
PubMed
Summary
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Epidermal growth factor (EGF) receptor mRNA levels increase in preovulatory follicles, correlating with decreased estradiol production. These high EGF receptor levels in the ovary may regulate estradiol production during ovulation.

Area of Science:

  • Reproductive biology
  • Endocrinology
  • Molecular biology

Background:

  • Epidermal growth factor (EGF) family members significantly inhibit estradiol-17 beta production in ovarian cells.
  • Increased binding of the EGF family to its receptor occurs during ovulation.

Purpose of the Study:

  • To quantify EGF receptor mRNA levels in preovulatory follicles.
  • To determine if EGF receptor mRNA levels remain elevated after functional luteolysis.

Main Methods:

  • Measurement of EGF receptor mRNA in whole ovaries, follicles, and corpora lutea using solution hybridization analysis.
  • Utilized pregnant mare serum gonadotrophin (PMSG)-stimulated rats for defined follicles and a pseudopregnant rat model for luteolysis.
  • Immunohistochemistry was employed to verify EGF receptor protein levels.

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Main Results:

  • A two-fold increase in EGF receptor mRNA was observed in preovulatory follicles between 09:00 and 21:00 on proestrus day.
  • Concurrently, serum estradiol-17 beta levels decreased significantly.
  • EGF receptor mRNA levels in the corpus luteum decreased from day 1 to day 11 post-ovulation.

Conclusions:

  • Elevated EGF receptor levels in the preovulatory follicle suggest a role in regulating ovarian estradiol-17 beta production.
  • The findings indicate a potential mechanism for controlling estradiol secretion during the ovulatory period.