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Immunohistochemical reference ranges for B lymphocytes in bone marrow biopsy paraffin sections
L R O'Donnell1, S L Alder, U J Balis
1Saint Mary's Medical Center, Saginaw, Michigan, USA.
American Journal of Clinical Pathology
|November 1, 1995
Summary
This study establishes normal reference ranges for B lymphocytes in bone marrow sections. These findings aid in diagnosing B-cell lymphoproliferative disorders using immunohistochemistry.
Area of Science:
- Hematology
- Immunology
- Pathology
Background:
- Quantifying B lymphocytes in bone marrow is crucial for diagnosing B-cell lymphoproliferative disorders.
- Established normal ranges for B lymphocytes in paraffin-decalcified bone marrow sections are lacking.
- Accurate quantitation requires reliable reference values for accurate diagnosis.
Purpose of the Study:
- To determine normal reference ranges for B lymphocytes in B5-fixed, paraffin-decalcified bone marrow sections.
- To provide essential data for the accurate immunohistochemical assessment of B-cell populations.
- To support the diagnosis of B-cell lymphoproliferative disorders.
Main Methods:
- Analysis of 34 normal bone marrow sections.
- Immunohistochemical staining using CD20, CD45RA (4KB5), DBA.44 for B cells, and CD45RO (UCHL-1) for T cells.
- Image analysis for cell quantitation.
Main Results:
- CD20-positive cells ranged from 0% to 5.97%.
- CD45RA positivity ranged from 0% to 5.48%.
- DBA.44 positivity ranged from 0% to 2.07%.
- CD45RO positivity ranged from 0% to 6.7%.
Conclusions:
- The study provides the first immunohistochemical reference ranges for B lymphocytes in B5-fixed, paraffin-decalcified bone marrow.
- These ranges are valuable for distinguishing normal B-cell populations from pathological ones.
- This research enhances diagnostic accuracy for B-cell lymphoproliferative disorders.