Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ca2+ currents in human colonic smooth muscle cells

Z Xiong1, N Sperelakis, A Noffsinger

  • 1Department of Molecular and Cellular Physiology, University of Cincinnati College of Medicine, Ohio 45267, USA.

The American Journal of Physiology
|September 1, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Long-term follow-up of complete Barrett's eradication endoscopic mucosal resection (CBE-EMR) for the treatment of high grade dysplasia and intramucosal carcinoma.

Endoscopy·2007
Same author

Severe intestinal graft-versus-host disease following autologous stem cell transplantation.

Bone marrow transplantation·2006
Same author

Regulation of ion channels in myocardial cells and protection of ischemic myocardium.

Heart failure reviews·2005
Same author

TP53 and gastric carcinoma: a review.

Human mutation·2003
Same author

Clinical characteristics of transmitted transfusion virus infection in children.

Journal of Tongji Medical University = Tong ji yi ke da xue xue bao·2003
Same author

Follicle-stimulating hormone (FSH) induced internalization of porcine FSH receptor in cultured porcine granulosa cells and Chinese hamster ovary cells transfected with recombinant porcine FSH receptor cDNA.

Journal of Tongji Medical University = Tong ji yi ke da xue xue bao·2003

This study investigated voltage-gated calcium channel currents in human colon smooth muscle cells. Researchers identified distinct fast and slow calcium current components with differing sensitivities to blockers, crucial for understanding colon muscle function.

Area of Science:

  • Physiology
  • Pharmacology
  • Cell Biology

Background:

  • Smooth muscle cells in the human colon (ascending and descending) possess voltage-gated calcium channels.
  • These channels play a critical role in regulating colonic smooth muscle contraction and motility.

Purpose of the Study:

  • To characterize the properties of voltage-gated calcium currents (ICa) in isolated human colon smooth muscle cells.
  • To differentiate between various components of ICa and their pharmacological sensitivities.

Main Methods:

  • Whole-cell voltage-clamp technique applied to freshly isolated smooth muscle cells from the human colon.
  • Investigation of ICa under varying holding potentials and in the presence of specific ion channel blockers (Ni2+, nifedipine, BAY K 8644).

Related Experiment Videos

Main Results:

  • Two distinct components of ICa were identified: a fast-inactivating and a slow-inactivating component.
  • The fast ICa component, observed at more negative potentials, was sensitive to Ni2+ but not nifedipine.
  • The slow ICa component, present across a wider range of potentials, was sensitive to nifedipine and BAY K 8644.

Conclusions:

  • Human colon smooth muscle cells exhibit complex voltage-gated calcium channel activity with at least two distinct functional components.
  • These components display differential sensitivity to pharmacological agents, suggesting the involvement of different calcium channel subtypes.
  • Understanding these ICa components is vital for comprehending colonic smooth muscle physiology and developing targeted pharmacological interventions.