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Related Experiment Videos

Nerve growth factor synthesis by intestinal epithelial cells

G W Varilek1, G A Neil, W P Bishop

  • 1Department of Internal Medicine, University of Kentucky College of Medicine, Lexington 40536-0084, USA.

The American Journal of Physiology
|September 1, 1995
PubMed
Summary

Intestinal epithelial cells synthesize and release nerve growth factor (NGF). This study demonstrates that these cells express NGF mRNA and secrete functional NGF, impacting neurite outgrowth.

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Area of Science:

  • Gastroenterology
  • Neuroscience
  • Cell Biology

Background:

  • Nerve growth factor (NGF) is present in the adult rat gut.
  • The specific cells responsible for NGF synthesis in the gut are currently unknown.

Purpose of the Study:

  • To investigate whether intestinal epithelial cells synthesize and release NGF.
  • To determine the role of intestinal epithelial cells in gut NGF production.

Main Methods:

  • Utilized IEC-6 and Caco-2 cell lines as models for intestinal epithelial cells.
  • Assessed NGF synthesis and release using conditioned media, neurite outgrowth assays, NGF-ELISA, and RT-PCR for NGF mRNA.
  • Investigated the effect of interleukin-1 on NGF secretion.

Main Results:

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  • Conditioned media from IEC-6 and Caco-2 cells promoted neurite outgrowth in PC-12 and DRG cells, which was partially blocked by anti-NGF antibody.
  • Immunoreactive NGF was detected in conditioned media, with low levels in cellular extracts.
  • NGF mRNA was expressed in IEC-6, Caco-2, and isolated rat intestinal epithelial cells.
  • Caco-2 cells secreted NGF primarily from the basolateral side, and interleukin-1 enhanced this secretion.

Conclusions:

  • Intestinal epithelial cells are capable of synthesizing NGF.
  • These cells contribute to the pool of NGF within the gut.
  • Intestinal epithelial cell-derived NGF may play a role in gut innervation and function.