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cAMP regulates gastrin gene expression

A Shiotani1, J L Merchant

  • 1Howard Hughes Medical Institute Research Laboratories, University of Michigan Medical Center, Ann Arbor 48109-0650, USA.

The American Journal of Physiology
|September 1, 1995
PubMed
Summary
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Cyclic AMP (cAMP) significantly boosts gastrin gene expression by increasing gastrin mRNA levels. This finding connects a key regulator of gastrin secretion to its gene expression control.

Area of Science:

  • Molecular biology
  • Gastroenterology
  • Endocrinology

Background:

  • Gastrin is a potent regulator of stomach acid secretion and gastrointestinal cell growth.
  • Gastrin release from antral G cells is controlled by various signals, but their effect on gastrin gene expression is unclear.
  • Gastrin gene expression changes significantly after birth, in response to achlorhydria, and in gastrinomas, indicating transcriptional regulation.

Purpose of the Study:

  • To investigate the effect of cyclic AMP (cAMP) on gastrin gene expression.
  • To determine if secretagogues that stimulate gastrin secretion also regulate its gene expression.

Main Methods:

  • Utilized stable and transient transformants of a pituitary cell line containing the human gastrin gene.
  • Analyzed the impact of cAMP on gastrin mRNA levels.

Related Experiment Videos

  • Mapped the regulatory elements responsive to cAMP within the human gastrin gene promoter.

    • Main Results:

    • cAMP treatment resulted in a three-fold increase in gastrin mRNA levels.
    • The cAMP-mediated response was localized to DNA elements between -148 and -40 base pairs upstream of the gastrin gene's cap site.
    • Established a link between cAMP, a regulator of gastrin secretion, and the transcriptional control of gastrin gene expression.

    Conclusions:

    • Cyclic AMP is a significant stimulator of gastrin gene expression.
    • The findings elucidate the transcriptional mechanisms underlying differential gastrin gene expression.
    • This research connects gastrin secretion regulation to gastrin gene expression regulation.