Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Increase of cardiac work is associated with decrease of mitochondrial NADH

J F Ashruf1, J M Coremans, H A Bruining

  • 1Department of General Surgery, Erasmus University, Rotterdam, The Netherlands.

The American Journal of Physiology
|September 1, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Alleviation of Post-sepsis Ischaemia by Drag-Reducing Polymers.

Advances in experimental medicine and biology·2024
Same author

Handheld vital microscopy for the identification of microcirculatory alterations in cervical intraepithelial neoplasia and cervical cancer.

Microvascular research·2023
Same author

The future of intensive care: the study of the microcirculation will help to guide our therapies.

Critical care (London, England)·2023
Same author

Real-time observation of microcirculatory leukocytes in patients undergoing major liver resection.

Scientific reports·2021
Same author

Can sidestream dark field (SDF) imaging identify subtle microvascular changes of the bowel during colorectal surgery?

Techniques in coloproctology·2018
Same author

Effects of Thoracic Epidural Anaesthesia on the Serosal Microcirculation of the Human Small Intestine.

World journal of surgery·2018
Same journal

Blood coagulation in fish.

The American journal of physiology·2011
Same journal

Renal tubular reabsorption, metabolic utilization and isomeric fractionation of lactic acid in the dog.

The American journal of physiology·2010
Same journal

The inactivation of placental toxin by human serum.

The American journal of physiology·2010
Same journal

Adrenal function following ovariectomy in the rat.

The American journal of physiology·2010
Same journal

Capillary permeability; perfusion of frog and guinea pig hind limbs.

The American journal of physiology·2010
Same journal

Evaluation of protective measures against sunburn.

The American journal of physiology·2010
See all related articles

Increased heart work reduces mitochondrial NADH/NAD+ ratios, indicating oxygen consumption changes drive, not follow, these redox shifts. This finding impacts understanding of cardiac energy metabolism.

Area of Science:

  • Cardiology
  • Mitochondrial Physiology
  • Cardiac Metabolism

Background:

  • Mitochondrial NADH/NAD+ ratio is a key indicator of cellular energy status.
  • The relationship between cardiac work, substrate supply, and mitochondrial redox state requires further elucidation.

Purpose of the Study:

  • To investigate the impact of cardiac work and substrate availability on the mitochondrial NADH/NAD+ ratio in isolated rat hearts.
  • To determine if changes in mitochondrial redox state precede or follow alterations in oxygen consumption during increased cardiac workload.

Main Methods:

  • Langendorff-perfused rat hearts were utilized to control physiological conditions.
  • Epicardial autofluorescence measured mitochondrial NADH/NAD+ ratio, with discontinuous UV excitation to prevent photobleaching.

Related Experiment Videos

  • Cardiac work was increased by elevating heart rate and left ventricular pressure, using nitroprusside to prevent vasoconstriction.

    • Main Results:

    • Increased oxygen consumption during substrate-limited (glucose) perfusion correlated with a significant decrease in NADH fluorescence (-31%).
    • Under pyruvate-rich conditions, elevated oxygen consumption also showed a significant decrease in NADH fluorescence (-20%).
    • These findings demonstrate a consistent decrease in the NADH/NAD+ ratio with increased workload and oxygen consumption.

    Conclusions:

    • The study suggests that an elevated mitochondrial NADH/NAD+ ratio is not the primary driver for increased cardiac respiration.
    • Changes in the mitochondrial NADH/NAD+ ratio appear to be a consequence, rather than a cause, of altered oxygen consumption during increased cardiac work.
    • This research provides critical insights into the regulatory mechanisms of cardiac energy metabolism.