Related Experiment Video
Updated: Aug 9, 2026
Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling
Published on: September 20, 2016
Simultaneous determination of itraconazole, hydroxy-itraconazole and amphotericin B in human plasma by HPLC with
C Lacroix1, F Wojciechowski, P Danger
1Laboratoire de pharmacocinétique, centre hospitalier, Le Havre, France.
Abstract:
A method for the fast analysis of itraconazole, hydroxy-itraconazole and amphotericin B is described which has the advantage of speed and specificity. The method is based on methanolic extraction of the compounds from plasma, followed by reverse-phase high-performance liquid chromatography with two detection wavelengths (263 and 405 nm). The extraction step provides > or = 94% recovery and the response of the detection system is linear from 50 to 5000 ng/ml. No other drugs have been found to interfere with the assay.
