Related Experiment Video
Updated: Aug 2, 2026
09:08
Retroviral Transduction of Bone Marrow Progenitor Cells to Generate T-cell Receptor Retrogenic Mice
Published on: July 11, 2016
Generation, translocation, and presentation of MHC class I-restricted peptides
1Center for Cancer Research, Massachusetts Institute of Technology, Cambridge 02139, USA.
Annual Review of Biochemistry
|January 1, 1995
Summary
Vertebrate T lymphocytes detect cellular changes by examining peptides presented by MHC molecules. The transporter associated with antigen presentation (TAP) moves peptides into the endoplasmic reticulum for MHC class I presentation.
Area of Science:
- Immunology
- Molecular Biology
- Cell Biology
Background:
- T lymphocytes survey the organism for changes by analyzing cell-surface peptides.
- Major histocompatibility complex (MHC) molecules present these peptides.
- MHC class I molecules present peptides derived from cytosolic proteins, processed by the proteasome.
Purpose of the Study:
- To describe the mechanism of peptide translocation into the endoplasmic reticulum for MHC class I presentation.
- To elucidate the role of the transporter associated with antigen presentation (TAP) in this process.
Main Methods:
- The study focuses on the molecular mechanisms of peptide transport and MHC class I assembly.
- It involves understanding protein interactions and cellular pathways.
Main Results:
- Peptides are translocated from the cytosol to the endoplasmic reticulum by the transporter associated with antigen presentation (TAP).
- TAP, composed of two subunits, specifically translocates peptides of 8-12 residues.
- The C-terminal residue of the peptide is crucial for TAP translocation specificity.
- Translocated peptides bind to MHC class I molecules, requiring chaperonins for proper folding.
- MHC class I-peptide complexes exit the ER for cell surface presentation.
Conclusions:
- The TAP transporter is essential for delivering peptides to MHC class I molecules.
- This process is critical for immune surveillance and T lymphocyte recognition of cellular changes.
Related Concept Videos
01:19Translocation of Proteins into the Mitochondria
Mitochondrial precursors are translocated to the internal subcompartments via independent mechanisms involving distinct protein machineries called translocases.
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...
01:22T Cell Activation and Clonal Selection
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
Naive T cells that have not yet encountered an antigen express two primary CD...