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Related Experiment Videos

Diversity of oligonucleotide functions

L Gold1, B Polisky, O Uhlenbeck

  • 1NeXstar Pharmaceuticals, Inc., Boulder, Colorado 80301, USA.

Annual Review of Biochemistry
|January 1, 1995
PubMed
Summary

SELEX technology identifies high-affinity oligonucleotide ligands from large random sequence libraries. These ligands target diverse molecules, enabling applications from basic research to diagnostics and therapeutics.

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Biotechnology

Background:

  • SELEX (Systematic Evolution of Ligands by Exponential Enrichment) is a powerful in vitro selection technology.
  • Oligonucleotides, particularly RNA and DNA, adopt complex, sequence-dependent folded structures in solution, exhibiting molecular rigidity.
  • This conformational complexity makes oligonucleotide libraries a rich source of high-affinity ligands.

Purpose of the Study:

  • To explain the principles and applications of SELEX technology.
  • To highlight the identification of high-affinity oligonucleotide ligands for diverse molecular targets.
  • To discuss the potential of SELEX in basic research, diagnostics, and therapeutics.

Main Methods:

  • SELEX involves generating large libraries of random sequence single-stranded oligonucleotides (RNA or DNA).
  • These libraries are subjected to iterative selection against specific molecular targets.
  • High-affinity binders are amplified and enriched through cycles of selection and amplification.

Main Results:

  • SELEX successfully identifies high-affinity ligands for a wide range of targets.
  • Identified targets include nucleic acid-binding proteins (polymerases, transcription factors), non-nucleic acid-binding proteins (cytokines, growth factors), and small molecules (ATP, theophylline).
  • The technology demonstrates broad applicability across various scientific domains.

Conclusions:

  • SELEX is a versatile technology for discovering high-affinity oligonucleotide ligands.
  • Its applications span basic research, novel diagnostic reagents, and therapeutic agents.
  • Future developments are expected to further enhance the utility and scope of SELEX.

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