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Related Experiment Videos

Spatial and temporal Ca2+ signalling in articular chondrocytes

P D'Andrea1, F Vittur

  • 1Dipartimento di Biochimica, Biofisica e Chimica delle Macromolecole, Trieste, Italy.

Biochemical and Biophysical Research Communications
|October 4, 1995
PubMed
Summary

Stimulating pig articular chondrocytes with various agents increased cytosolic calcium (Ca2+). This calcium response originated from intracellular stores and spread throughout the cell, showing significant cell-to-cell variability in calcium storage.

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Area of Science:

  • Cell Biology
  • Biochemistry
  • Physiology

Background:

  • Articular chondrocytes play a crucial role in maintaining joint health.
  • Calcium signaling is fundamental to cellular processes in chondrocytes.
  • Understanding calcium dynamics is key to addressing cartilage pathologies.

Purpose of the Study:

  • To investigate the spatial and temporal dynamics of cytosolic calcium (Ca2+) in pig articular chondrocytes.
  • To determine the source of calcium increase (intracellular vs. influx).
  • To explore the heterogeneity of calcium responses within the chondrocyte population.

Main Methods:

  • Stimulation of chondrocytes with bradykinin, fetal calf serum, and thapsigargin.
  • Computerized video imaging for real-time Ca2+ concentration analysis.

Related Experiment Videos

  • Single-cell level analysis of spatial and temporal Ca2+ signaling patterns.

    • Main Results:

    • Stimuli induced significant increases in cytosolic Ca2+ concentration.
    • Calcium originated primarily from intracellular stores, with minimal extracellular influx.
    • A marked heterogeneity in Ca2+ storage capacity was observed among chondrocytes.
    • Ca2+ signals initiated in specific subcellular regions and propagated throughout the cytosol.
    • Spatially restricted, pulsatile Ca2+ spikes were detected at the initiation sites.

    Conclusions:

    • Pig articular chondrocytes exhibit complex, heterogeneous calcium signaling responses.
    • Intracellular calcium stores are the primary source for stimulated Ca2+ release in these cells.
    • The observed signaling patterns suggest functional heterogeneity in intracellular calcium stores.