Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A gene-targeting suicide vector for Streptococcus bovis

J D Brooker1, D K Lum, A M Thomson

  • 1Department of Animal Science, Waite Agricultural Research Institute, University of Adelaide, SA, Australia.

Letters in Applied Microbiology
|November 1, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Impact of blood flow restriction training timing: Does exercising at dusk or dawn enhance response?

Physiological reports·2025
Same author

Bridging the age gap in breast cancer: impact of omission of breast cancer surgery in older women with oestrogen receptor-positive early breast cancer on quality-of-life outcomes.

The British journal of surgery·2021
Same author

Exploration of the perceptions, barriers and drivers of pharmacogenomics practice among hospital pharmacists in Adelaide, South Australia.

The pharmacogenomics journal·2013
Same author

Maternal stature and reproductive efficiency.

The Eugenics review·2011
Same author

A feeding experiment among Indian Army recruits.

The Indian journal of medical research·2010
Same author

Post-partum Retinal Arterial Obstruction with Hemiplegia.

British medical journal·2010

Researchers developed a gene-targeting suicide vector for Streptococcus bovis, enabling genetic manipulation of ruminal bacteria. This breakthrough facilitates gene knockout or introduction, potentially improving animal production.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Ruminant Nutrition

Background:

  • Streptococcus bovis is a key ruminal bacterium.
  • Genetic manipulation tools for S. bovis are limited.
  • Understanding ruminal microbial function is crucial for animal production.

Purpose of the Study:

  • To construct and characterize a novel gene-targeting suicide vector for Streptococcus bovis.
  • To establish a recombination system for genetic manipulation in ruminal bacteria.
  • To enable targeted gene modification in S. bovis for potential applications in animal production.

Main Methods:

  • Construction of a suicide plasmid using an E. coli/Streptococcus shuttle vector and Tn916 transposon.
  • Testing plasmid replication and integration in S. bovis.

Related Experiment Videos

  • Positive selection for plasmid integration into chromosomal DNA.
  • Assessing plasmid stability over multiple generations.

    • Main Results:

    • A functional gene-targeting suicide vector for S. bovis was successfully constructed.
    • The plasmid replicated in E. coli but not in S. bovis.
    • Stable integration into S. bovis chromosomal DNA was achieved at a frequency of 3 x 10(-8) cell-1.
    • The integrated plasmid was stably maintained for over 100 generations without selection.

    Conclusions:

    • This study reports the first recombination system for ruminal bacteria, specifically S. bovis.
    • The developed suicide vector allows for targeted gene manipulation (knockout or introduction) in S. bovis.
    • This technology has the potential to advance the understanding and manipulation of ruminal species, possibly leading to improved animal production.